Effect of Adenine on Viability of Human Red Cells Preserved in the Frozen State

摘要

The addition of adenine, in amounts sufficient for a final concentration of 0.5 micromoles per ml. of blood, had no beneficial effect on the ability of ACD or CPD bloods to withstand the trauma incident to freezing by the glycerol technique, processing being carried out in the Cohn-ADL Fractionator. Analysis of significant osmotic and metabolic changes in red cells so processed have provided a basis for treating frozen cells processed in a new simplified centrifuge (Latham Bowl) to minimize damage. The addition of adenine to red cells of CPD blood at the time of resuspension in autologous plasma, results in maintenance of viability above the minimal permissible limit of 70% for 21 days. These findings indicate that bloods frozen by the technique described may be integrated with the normal procedures of current blood bank practice. (Author)