(32)P-Postlabeling Analysis of DNA Adducts of Two Nitrated Polycyclic Aromatic Hydrocarbons in Rabbit Tracheal Epithelial Cells

摘要

The 1-nitropyrene (1-NPP and 3-nitrofluoranthene (3-NF) adducts have been analyzed by (32)P-postlabeling and with 1-NP have been compared to the total number of adducts estimated from (14)C binding in rabbit trachael epithelial (RTE) DNA samples. One adduct spot, by (32)P-postlabeling, was detected in the DNA of RTE cells treated with (14)C-1-NP. The adduct, at approximately 6 adducts 10-to the 9th power nucleotides, represents a minor component of the binding to RTE DNA being lesser than 1% of the total (14)C-radioactivity associated with the extracted DNA. Treatment of RTE cells with unlabeled 3-NF resulted in two adduct spots at approximately 12 adducts/10 to the 9th power. When the P1 nuclease treatment was used as an alternative method both the 3-NF derived adducts previously detected following the butanol extraction were absent.