CHROMATOGRAPHIC BEHAVIOUR AND CHEKIICAL STRUCTURE. I.THIN-LAYER CHROMATOGRAPHY OF ALIPHATIC ACIDS.

摘要

About sixty aliphatic acids, predominantly fatty acids, hydroxy acids, amino acids and halogen acids, ware separated on cellulose layers with the solvent mixture n-butanole - diethylamine - water (85:1:14). The Rm values, thus obtained exhibit in most cases the validity of filartin's theoretical postulates for the relationship between chemical structure and Rf value in partition chromatography. Thus when Rm is plotted against the number of substituent groups of any one kind a close approximation to linearity results, except in the case of acids with vicinal hydrophilic groups. The calculation of group constants and binding increments was done by direct comparison of the Rm values of substances differing by only one group or increment. These data facilitate chromatographic elucidation of structural problems requiring only very small substances not even isolated in pure form.