Chain Heterogeneity as Demonstrated by the Ferricyanide Oxidation of the One-Site Reduced Bovine Methemoglobin. Progress Report, December 1, 1977--November 30, 1978

摘要

With the pulse radiolysis technique, a single chain of tetrameric bovine methemoglobin ( alpha sub 2 /sup III/ beta sub 2 /sup III/) is reduced, producing a mixture of valence hybrids consisting of alpha /sup III/ alpha /sup II/ beta sub 2 /sup III/ and alpha sub 2 /sup III/ beta /sup III/ beta /sup II/. The oxidation of these partially reduced hemoglobins with ferricyanide was studied. It was found that the rate of oxidation of one of them proceeds 6 to 11 times faster than the other. It is suggested that the fast reacting valence hybrid contains reduced alpha chains. (ERA citation 04:024107)