Role of Ca exp 2+ and Calmodulin in Phosphorylation of Proteins in Plants. Progress Report.

摘要

The goal was to provide an understanding, at the molecular level, of the in vitro requirements for Ca exp 2+ -dependent protein phosphorylation in plant cells and to ascertain whether this Ca exp 2+ -mediated phosphorylation represents one of the underlying mechanisms by which plants respond to external stimuli such as heat shock, growth regulators, etc. The first 10 months of this granting period resulted in (1) setting up a tissue culture system for growing soybean cells in large quantity from which protein kinases and their substrates may be isolated, (2) demonstrating the existence of Ca exp 2+ -dependent protein kinases in extracts of these soybean cells, (3) demonstrating the presence of a number of endogenous substrates for these Ca exp 2+ -dependent protein kinases, and (4) developing a rapid and sensitive assay for calmodulin. 4 figures, 3 tables. (ERA citation 09:038483)