Development of a Genetic System in Bacteroides. Final Report, June 14, 1981-June 14, 1985

摘要

We have constructed a chimeric plasmid, pE5-2, that can be mobilized from E. coli to Bacteroides by the broad host range plasmids R751 and RK2. pE5-2 is maintained in Bacteroides and carries a gene for resistance to erythromycin-clindamycin that is expressed in Bacteroides. We have discovered and described the first Bacteroides transposon, Tn4351 and we have shown that this transposon also mediates the integration of R751 into the chromosome. This feature may serve as a basis for future construction of Hfr-type strains of Bacteroides. We have cloned and characterized a regulated Bacteroides gene that codes for a polysaccharidase (chondroitin lyase). The cloned DNA appears to contain the Bacteroides promoter. Finally, we have developed a method for rapid identification of some Bacteroides species, using species-specific cloned DNA fragments. These accomplishments provide for the first time a basis for genetic analysis of human clonic Bacteroides. 5 refs., 3 figs. (ERA citation 10:040164)