One Carbon Metabolism in Anaerobic Bacteria: Organic Acid and Methane Production. Final Technical Report

摘要

Organic acids (i.e acetate and butyrate) and methane are respectively important as a chemical commodity and a fuel source for industry. Both of these products can be generated via bacterial fermentation of single carbon compounds (i.e, H sub 2 /CO sub 2 , HCOOH, CO CH sub 3 OH) derived from syngas or the pyrolysis of either coals-peats or renewable biomass. This research aims to understand the pathways and regulation of one carbon metabolism in acidogenic and methanogenic bacteria by detailed physiological and biochemical studies. These investigations will characterize and compare formate, methanol, H sub 2 /CO sub 2 , CO, and acetate metabolism of Methanosarcina barkeri with that of Butyribacterium methylotrophicum. The research will focus on: elucidation of the function of formate dehydrogenase and carbon monoxide dehydrogenase; the catabolic routes and biochemical mechanisms for transformation of single carbon compounds and acetate; and, the regulation of single carbon metabolism during growth on multiple C sub 1 substrates or on multicarbon substrates. 8 refs. (ERA citation 11:011129)