Charge Standards as Reference Points in Two-Dimensional Western Blot Patterns

摘要

High-resolution two-dimensional electrophoresis can be used to separate hundreds or even thousands of proteins from complex mixtures. Specific proteins in 2DE patterns can be identified by using Western Blotting. Correlation between a single stained spot on a nitrocellulose blot and the same spot in a stained 2DE gel pattern can be difficult since no reference points are available on the blot. Inclusion of a protein or proteins to serve as reference or standard points in both the blotted pattern and the stained gel pattern would make pattern recognition much more rapid and reliable. In this paper the use of a commercial charge standard (carbamylated creatine phosphokinase mix) was used to produce reference points in both gel patterns and corresponding nitrocellulose blots to simplify recognition of individual protein spots in complicated gel patterns. 8 refs., 2 figs. (ERA citation 12:019130)