Estimation of DNA synthesis in lymphocyte as biological indicator in radiation accidents. Final report for the period November 1, 1986 - November 30, 1988.

摘要

The IAEA-supported research contract 4535 entitled ''Estimation of DNA synthesis in lymphocyte as biological indicator in radiation accidents'' has been successfully carried out by the investigators at the Institute of Radiation Medicine in Beijing, People's Republic of China. A new potential trend has been fostered and enhanced through the reported work to add to the growing spectra of quantitative biological dosimetry systems for absorbed radiation exposure monitoring in aid of radiation protection services. The current topic deals with assay system based on the body's intrinsic repair capacity for radiation-induced genetic (DNA) lesions. Unscheduled DNA synthesis (UDS) induced by ultraviolet light has been calibrated and quantitated in vitro to measure cellular repair capacity. Dose-dependent detriments of this repair capacity in both lymph nodes and peripheral blood lymphocytes following whole- and part-body exposure(s) to /sup 60/Co-gamma radiation have been estimated using current techniques of molecular radiation cytogenetics and relevant analytical methods. Lymphocyte transformation assay to determine the quantitative effects of radiation exposure doses has also been attempted and some preliminary data have been reported. The method, however, is at present of somewhat lesser precision but holds promises for the future. 4 refs, 1 fig., 7 tabs. (Atomindex citation 20:045224)