Development of a novel sensor for bioreactor operation

摘要

For a batch bioreactor operation, the bioluminescence emission profile can be used as an indication for the metabolic state of the cells, because of its high sensitivity to environmental perturbations, such as a change in temperature, pH, the dissolved oxygen level, and inhibitory factors. Among the twelve experiments several qualitative correlation features between the light emission and the biomass growth profiles were observed. However, a consistent quantitative correlation cannot be obtained, mainly because of the inconsistency of the level of emitted light from batch to batch. We are currently trying to control the operation more tightly to see if we,can obtain a more consistent output. However, we plan to switch to the continuous mode of operation, thus controlling the growth to be exponential.