DEPSCoR 99 Percutaneous Absorption, Skin Biophysics and Dermatotoxicity from JP-8 Jet Fuel

摘要

JP-8 and its major aromatic and aliphatic components were investigated for percutaneous absorption and dermal toxicity in vitro and in vivo. In vitro studies showed SC lipid and protein extraction, macroscopic barrier perturbation and microscopic changes in JP-8 treated skin samples. Transmission electron micrograph showed many ultrastructural modifications in JP-8 treated porcine skin namely expansion in the intercellular spaces of stratum comeum, reduction in compactness of tonofibrils of stratum spinosum, altered configuration and disrupted attachment of basale cells, reduction in dendritic processes of Langerhans' cells. In in vitro studies with JP-8 components, tridecane exhibited greater permeability through skin among aliphatic and naphthalene among aromatic JP-8 components, which may be due to their greater diffusion through skin. All of the chemicals caused significant (p <0.05) increase in TEWL values through skin. This indicates potential skin irritation and dermatotoxicity by JP-8 components. Amount of chemicals absorbed suggests that tridecane among aliphatic components and, naphthalene and its methyl derivatives among aromatic components should be monitored for their possible systemic toxicity. In viva studies with JP-8 and its model components in rabbit showed an Increase in temperature, capacitance, TEWL and severe to moderate erythema and edema with all test chemicals. The quantitative microscopic measurement results showed increase in epidermal thickness, and decrease In collagen fibers' bundle length and thickness. This constitutes potential dermatotoxicity from these chemicals. The DEPSCoR funding also allowed us to provide research experience to a research associate. a graduate student, and two undergraduate students in the area of percutaneous absorption and dermal toxicity from JP-8 and its major components and led to six publications in peer- reviewed journals and ten presentations In national/international symposia and seminars.