Host and Environmental Factors Influencing the Manifestation and Propagation of the Yeast Prions

摘要

The artificial prion PSI+ based on a hybrid Sup35PS with prion domain from the yeast Pichia methanolica PSI+(ps) was used to search a multicopy Saccharomycetes cerevisiae genomic library for novel factors able to cure prions. It was found that overexpression of the Hsp40 family chaperones Sis1 and Ynl077w, chaperone Sti1, transcriptional factors Sfl1 and Ssn8 and acidic ribosomal protein Rpp0 interfere with propagation and manifestation of the PSI+prion. This implies that similar factors may be active against human and animal prion and amyloid diseases.