In Vitro Toxicity of Nanoparticles in Mouse Keratinocytes and Endothelial Cells

摘要

This study was undertaken to assess the potential toxicity of nanomaterials in mouse keratinocytes (HEL-30) and endothelial cells (bEnd.3). The nanoparticles tested were aluminum (Al - 30 nm), silver (Ag - 15 nm hydrocarbon coated, 100 nm uncoated), and molybdenum trioxide (MoO3 -30 nm). For toxicity evaluations, mitochondrial function (MU assay), lysosomal membrane integrity (NR assay) and cellular morphology were assessed under control and exposed (10-250 mg/ml) conditions for an exposure period of 24 hours. In the MN assay, the 15 nm Ag particles were found to be highly toxic in HEL-30 cells when compared to Al-30 nm and MoO3-30 nm particles, which produced no toxicity at the tested concentrations. The 100 nm Ag particles also did not produce significant toxicity in HEL-30 cells. However, Ag-100 nm particles did induce toxicity in bEnd.3 cells. bEnd.3 cells were also three times more sensitive to Ag-IS as compared to HEL-30 cells. Like HEL-30 cells, the bEnd.3 cell line displayed no toxicity in the presence of AI-30 nm or MoO3-30 nm particles. NR uptake data in HEL-30 cells also confirmed that Ag-IS nm particles were highly toxic compared to Al and MoO3 nanoparticles. Observation using a phase contrast inverted microscope indicated that increased concentration of Ag-IS led to a change in cell morphology. Cells affected by nanoparticle exposure showed a decrease in cellular volume and a change in cell shape. Further comparisons of other nanomaterials are planned using in vitro cells originatin from pulmonary, liver and skin tissues.