Contact Versus Noncontact Cell Electroporation

摘要

In this research, experiments and simulations were performed to determine the effects of capacitive VS conductive coupling for the electroporation of cells. The experiment is performed with a fast risetime pulser to expose samples of cells to electric fields at peak voltage of 24.4 kV, 0.6 ns risetime, and 1.6 ns FWHM. Experiments performed compare the direct- conductive connection of the cell suspension versus a capacitively coupled cell suspension. The main conclusion of this research is that, for the parameters of this experiment, electroporation does not occur for capacitive coupling (Displacement Current): Conduction current is required for electroporation to occur.