Factors Affecting the Level of Infectivity of RNA of Venezuelan Equine Encephalomyelitis (VEE) Virus.

摘要

RNA of the Venezuelan equine encephalomyelitis (VEE) virus, isolated from virions and infected cells was studied, and the main parameters for detection of maximal infectivity of the viral RNA were determined. It was shown that, among the factors determining the RNA infectivity level, adsorption time of the RNA on cells, the concentration and duration of treatment of the cells with NaCl, and the addition of polycations (DEAE-dextran, protamine sulfate) to the agar overlay, are all of significant importance. The data obtained in the study suggest optimal conditions for determining the infectious activity of the RNA of the VEE virus. (Author)