Rodent Bone Marrow Micronucleus Assay. Test Substance: Solvent Yellow 33 2-(2-Quinolyl)-1,3-indandione

摘要

Solvent Yellow 33 2-(2-Quinolyl)-1,3-indandione (CAS No. 8003-22-3), was tested for its ability to induce increase in the frequency of micronuclei in the bone marrow erythrocytes of mice. The test was dosed to mice orally at 2000, 666, and 222 mg/kg body weight in corn oil as a single dose (OECD 474 guidelines). Bone marrow smears were prepared from the test and negative control groups at 24 and 48 hours posttreatment. Bone marrow smears for the positive control group were prepared at 24 hours post treatment. Polychromatic (PCE) and normochromatic (NCE) erythrocytes were scored from these bone marrow smears and were examined for the presence of micronuclei for evaluation of clastogenicity. There was no statistically significant increase in the frequency of micronucleated PCEs in the test substance dose groups as compared to the negative control group. The positive control group caused a statistically significant increase in micronucleated cells as compared to the negative control group. Based on the criteria of the study protocol, the test substance is considered nonclastogenic, under the experimental conditions.