Meningococcal Control in the Canadian Forces II. Assessment of Growth-Supporting Media for the Transport of 'Neisseria meningitidis'.

摘要

The abilities of Transgrow (TG), Thayer-Martin (TM) and New York City (NYC) solid media to maintain viability of 12 strains of Neisseria meningitidis under various controlled conditions were assessed. The effects of charcoal impregnation of swabs, temperature and the presence of CO2 were determined with holding for up to 21 days. Strains employed included 5 laboratory strains, 3 isolated from the nasopharynx of apparently healthy carriers and one isolated from a naturally occurring aerosol. Recovery from those samples held at 35 C was in almost all instances greater than at 22 or 4 C. A strong requirement for CO2 was demonstrated, especially at lower temperatures. No positive effect could be attributed to the use of charcoal-impregnated swabs. NYC and TM media were found to be the best overall, with the former permitting recovery from more than 75% of all samples held on slants after holding for 20 days at 4 C in the presence of 5% CO2. (Author)