Role of Cellular Components of Mosquito Cells in Viral Replication and Transmission

摘要

Several mosquito cell proteins have been identified as being associated with (or incorporated in) Banzi virus grown in the polyclonal cell line of A. albopictus cells. This fact has been demonstrated by several observations. First, Banzi virions purified by polyethylene glycol precipitation and equilibrium density sucrose gradient centrifugation contain four mosquito cell protein. Second, anti-mosquito cell serum aggregates mosquito cell-grown Banzi virus, but not Banzi virus propagated in BHK cells. Third, anti-mosquito cell serum blocks the agglutination of erythrocytes by purified Banzi virus. Uninfected mosquito cells secrete a complex of proteins which is able to agglutinate goose erythrocytes. The density of the complex and the pH optimum of its hemagglutinating activity are different than that of Banzi virus grown in mosquito cells for 24 hours. Upon prolonged infection of mosquito cells with Banzi virus, the progeny virions differ in several respects. An apparent increase in the amount of one cellular protein is accompanied by a change in the pH optimum of agglutination of erythrocytes by Banzi virus. As a result of the incorporation of mosquito cell proteins into Banzi virions, mice immunized with uninfected mosquito cells or with purified A. albopictus hemagglutinin are protected against death due to Banzi viral infection.