Morphologic Accounts of a Human Skin Equivalent Exposed to Mustard Gas

摘要

TESTSKIN, a commercially available human skin equivalent, was used as a modelsystem to study the temporal anatomical-pathological effects of a single vesicating vapor dose of sulfur mustard (HD). Samples were exposed to 10 ul HD vapor for 8 min and harvested at 1, 3, 6, 12, and 24 hr following exposure. Control samples not exposed to HD were harvested at 0 and 24 hr. light and electron microscopic analysis revealed that the basal cell of the stratum germinativum was selectively affected beginning at 36 hr. These early basal cell changes included, an apparent widening of intercellular spaces; a disabling of desmosomal attachments; rounding of cells, nuclear condensations, and pyknosis; rearrangement of cytoplasmic tonofflaments to a perinuclear position; and perinuclear blebbing. At 12 and 24 hr, the cytopathology progressed to cytoplasmic vacuolation, swollen endoplasmic reticulum, electron opacities, and necrosis that now involved suprabasal cell layers as well. At the basement membrane zone, cellular debris and cellular fragments accumulated in the area of the lamina lucida that appeared to widen this space, resulting in the formation of a cleft. These results are largely consistent with those reported for animal models and cells in culture. In the course of this morphologic study it was observed that skin structures normally present in vivo were absent or incomplete in the human skin equivalent specimens. These included hemidesmosomes, a basement membrane, anchoring filaments, and anchoring fibrils.