Enhanced Osteoblast Differentiation on Scaffolds Coated with TiO_2 Compared to SiO_2 and CaP Coatings

摘要

The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO_2, SiO_2 and calcium phosphate (CaP); Ca_9HPO_4(PO_4)_5OH, were applied to porous TiO_2 scaffolds prepared by polymer sponge replication. NHO were cultured on scaffolds in triplicates. The concentration of cytokines and Ca~(2+), and alkaline phosphatase (ALP) activity in the cell media was quantified. The secretion of osteopontin, osteoprotegerin, vascular endothelial growth factor and interleukin-6 was higher from NHO on TiO_2 compared to SiO_2 and CaP. The secretion from cells on the three scaffolds was, however, either similar or lower than the control cells cultured on plastic. The Ca~(2+) concentration was higher in cell media on CaP the first week, and no difference in ALP activity was observed. TiO_2 coating induced a higher secretion of factors indicating enhanced osteoblast differentiation as compared to CaP and SiO_2.