Microgel Assembly Powder Improves Acute Hemostasis, Antibacterial, and Wound Healing via In Situ Co-Assembly of Erythrocyte and Microgel

摘要

Rapid and effective hemostasis on irregular-shaped non-compressiblebleeding wounds is still one of the key challenges in clinical practice. Herein, anovel multi-functional microgel assembly powder (MAP) composed ofoxidized dextran/methacrylate gelatin (ODex/GelMA) microgel powder andlong-chain alkyl quaternized chitosan (LQCS) crosslinker powder is reportedfor acute hemostasis, antibacterial, and wound healing. When MAP is appliedonto bleeding wounds, porous ODex/GelMA microgel powder immediatelyabsorbs massive liquid in blood whilst LQCS induces in situ co-assembly oferythrocytes and microgels. In other words, MAP combines the merits ofhemostatic powders and hemostatic hydrogels, that is, the excellenthemostasis performance of MAP is originated from not only the high fluiduptake ratio/rate that causes the aggregation of coagulation factors anderythrocytes, but also the formation of erythrocytes-involved hydrogel-likemicrogel assembly that dramatically improves the mechanical strength, tissueadhesion and wound sealing performance in blood compared with those inPBS. In addition, MAP also exhibits excellent antibacterial ability, andpromotes the wound healing in bacterial-infected full-thickness skin wounds.The fantastic features of MAP including low cost, easy synthesis and usage,excellent hemostasis on irregular-shaped, non-compressible bleedingwounds, outstanding antibacterial and wound healing, good cytocompatibility,make it a promising hemostatic material and wound dressing.