PRODUCTION OF CAMPTOTHECIN FROM ENDOPHYTIC FUNGI AND CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ANTICANCER ACTIVITY AGAINST COLON CANCER CELL LINE.

摘要

Objective: Scientists are showing increasing interest in studying fungal endophytes as sources of novel and biologically active compounds. The present study was attempted to isolate and characterize camptothecin (CPT) from endophytic fungus Aspergillus niger isolated from Indian Piper betel plant and detection of its anticancer potential on colon cancer cell line. Methods: The production of CPT was confirmed by high-performance liquid chromatography techniques. The effect of CPT on hematopoietic cell transplantation (HCT) colon cancer cell line was studied by MTT assay. Results: The amount of CPT which is isolated from A. niger was found to be around 0.175 mg/L . Significant death of HCT cells was observed and was non-toxic to normal vero cell line. Conclusion: Hence, CPT can be used as a potential lead compound in cancer research.