Determination of iriflophenone 3-C- beta-D-glucoside from Aquilaria spp. by an indirect competitive enzyme-linked immunosorbent assay using a specific polyclonal antibody.

摘要

Polyclonal antibody against iriflophenone 3-C- beta-D-glucoside (IP3G), a major compound from the leaves of Aquilaria spp., was produced for the development of an enzyme-linked immunosorbent assay (ELISA). The results showed that the antibodies were specific for IP3G. The produced antibody has low cross reactivity with iriflophenone 3,5-C- beta-D-diglucopyranoside (13), genkwanin 5-O- beta-primeveroside (3.55) and no cross reactivity found in other compounds. The range of ELISA assay extends from 100 to 1560 ng/mL with coefficient of variation (CV) 1.19 to 2.07 for intra-assay and 3.76 to 7.15 for inter-assay precision levels. The recovery rates of IP3G in the leaves of Aquilaria spp. were in the range of 96.0 to 99.0 with CV 4.50 to 5.32. A correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of IP3G in the plant samples (R 2 = 0.9321). These results suggest that the developed ELISA method can be applied to determine IP3G content with high specificity, rapidity, and simplicity. The developed immunosorbent assay in this study provides a useful tool for the analysis of IP3G in plant samples and products. Practical application. Agarwood leaves are consumed as health drink due to their proposed anti-inflammatory, anti-diabetic properties, and as a laxative. An indirect competitive enzyme-linked immunosorbent assay method for determination of iriflophenone 3-C- beta-D-glucoside showed potential assay for quality control of agarwood leaves raw material and product. copyright 2013 Institute of Food TechnologistsReg..