Adipogenic potential of perivascular adipose tissue preadipocytes is improved by coculture with primary adipocytes

摘要

Perivascular adipose tissue (PVAT) has the capacity to secrete vasoactive mediators with the potential to regulate vascular function. Given its location adjacent to the vasculature, PVAT dysfunction may be part of the pathophysiology of cardiovascular diseases. To study the mechanisms of PVAT dysfunction, several adipogenic models have been proposed. However, these approaches do not adequately reflect PVAT adipocyte phenotypes variability that depends on their anatomical location. Despite PVAT importance in modulating vascular function, to date, there is not a depot-specific adipogenic model for PVAT adipocytes. We present a model that uses coculturing of PVAT stromal vascular fraction derived preadipocytes with primary adipocytes isolated from the same PVAT. Preadipocytes were isolated from thoracic aorta PVAT and mesenteric resistance artery PVAT (mPVAT). Upon confluency, cells were induced to differentiate for 7 and 14days using a standard protocol (SP) or standard protocol cocultured with primary adipocytes isolated from the same adipose depots (SPA) for 96, 120, and 144h. SPA reduced the time for differentiation of stromal vascular fraction derived preadipocytes and increased their capacity to store lipids compared with SP as indicated by lipid accumulation, lipolytic responses, gene marker profile expression, and adiponectin secretion. The coculture system improved adipogenesis efficiency by enhancing lipid accumulation and reducing the time of induction, therefore, is a more efficient method compared to SP alone.