An RNA aptamer with potent affinity for a toxic dimer of amyloid?42 has potential utility for histochemical studies of Alzheimer's disease

摘要

Oligomers of ?-amyloid 42 (A?42), rather than fibrils, drive the pathogenesis of Alzheimer's disease (AD). In particular, toxic oligomeric species called protofibrils (PFs) have attracted significant attention. Herein, we report RNA aptamers with higher affinity toward PFs derived from a toxic A?42 dimer than toward fibrils produced from WT A?42 or from a toxic, conformationally constrained A?42 variant, E22P?A?42. We obtained these RNA aptamers by using the preincubated dimer model of E22P?A?42, which dimerized via a linker located at Val-40, as the target of in vitro selection. This dimer formed PFs during incubation. Several physicochemical characteristics of an identified aptamer, E22P?AbD43, suggested that preferential affinity of this aptamer toward PFs is due to its higher affinity for the toxic dimer unit (K-D = 20 ? 6.0 nm) of A?42 than for less-toxic A?40 aggregates. Comparison of CD data from the full-length and random regions of E22P?AbD43 suggested that the preferential binding of E22P?AbD43 toward the dimer might be related to the formation of a G-quadruplex structure. E22P?AbD43 significantly inhibited the nucleation phase of the dimer and its associated neurotoxicity in SH-SY5Y human neuroblastoma cells. Of note, E22P?AbD43 also significantly protected against the neurotoxicity of WT A?42 and E22P?A?42. Furthermore, in an AD mouse model, E22P?AbD43 preferentially recognized diffuse aggregates, which likely originated from PFs or higher-order oligomers with curvilinear structures, compared with senile plaques formed from fibrils. We conclude that the E22P?AbD43 aptamer is a promising research and diagnostic tool for further studies of AD etiology.