Newly Designed Primers for Amplifying and Sequencing 16S Ribosomal Mitochondrial DNA Gene for Vertebrates

摘要

The complete sequence of 16S rRNA gene for 54 diverse vertebrate taxa, in the present study, was collected from the Genbank database and they were manually aligned. From the resulted alignment, the conserved regions were searched and new conserved universal primers were designed. The reliability of these primers was tested and confirmed by amplifying and sequencing the targeted gene for taxa that are belonging to diverse vertebrate classes. This method helped in establishing a new set of primers to accelerate both the amplification and the sequencing of vertebrate mitogenomes. It also aids in building molecular markers for studying the genetic framework of different vertebrate taxa as a preliminary strategy for their biodiversity.