Synthesis and Characterization of Novel Calix4arene Schiff Base Derivatives and Cytotoxicity Effect Evaluation on Cancer Cell Lines

摘要

In this study, four stages were used to create brand-new p-tert-butyl-calix 4 arene Schiff base derivatives. First, p-tert-butyl-phenol and formaldehyde are reacted to create p-tert-butyl-calix 4 arene (1). In the following step, methyl bromoacetate and p-ter-butyl-calix 4 arene (1) were combined with acetone and reflux to create the p-tert-butyl-calix 4 arene diester complex (2). The third step involves reacting the diester compound (2) and hydrazine hydrate to create the p-tert-butyl-calix 4 arene hydrazinamide molecule (3). In the final stage, calix 4 arene Schiff base derivatives (4a-d) were produced in good yields by combining compound (3), p-tert-butyl-calix 4 arene hydrazinamide, and various aldehyde derivatives with reflux in EtOH. Through the use of 1H-NMR, 13C-NMR, infrared spectroscopy, and elemental analysis, the structures of produced compounds were verified. Four distinct cancer lines are linked to the antitumor activity of synthetic chemicals. (HT-29, a human colon cancer cell line, PC-3, a human prostate cancer cell line, C6, a rat glioma cell line and MCF-7, a human breast cancer cell line). Weak antitumor activity was seen in synthetic substances. However, only compound 4b was found to have potential efficacy against C6 and HT-29. It is clear that compound 4b, which has a nitro substitute on the phenyl ring, draws attention due to its increased activity.