EFFECTS OF ULTRASOUND ON ARTEMIA CYST INCUBATION

摘要

The present work aims to test the bioeffects of ultrasounds on cyst formation in the crustacean Artemia salina. It is designed to identify which durations of ultrasonic exposure are capable of in?encing hatching rates. The transducers used in the experiment were operated at resonant (0.23MHz and 4.7 MHz) and non-resonant (0.5MHz and 1 MHz) frequencies and the intensities of 3.25mW/cm~2 and 13.63mW/cm~2. The Rayleigh-Plesset theoretical framework was used to calculate the dynamics of shell-encapsulated cysts. The duration of ultrasound exposure was approximately 2 min every 2 h, repeated 6 times in the culture range of 1-15 h. The results demonstrate that irradiation at an intensity of 13.63mW/cm~2 and a driving frequency of 0.23MHz increased the number of hatched larvae by approximately 31.7, compared with unexposed control samples. In conclusion, the theoretical model provided a useful means of predicting the most e±cient frequency of sonication.