Anticancer activity of Gloriosa superba Linn in Ehrlich ascites carcinoma tumor-bearing Balb/c mice

摘要

Cancer is a pathogenic condition due toabnormal growth of cells, which is one of the leading reasonsfor the death of majority of the population throughout theworld. Pharmacotherapy associated with the majority of theadverse effects such as cardiotoxicity, hepatotoxicity,nephrotoxicity that suggests identifying new drug whichwould be safer than well-established chemotherapy. There isa need to get a promising line for research over variousdiseases including cancer. Gloriosa superba Linn has a longhistory of use in folk medicine including wounds,hemorrhoids, kidney problems, and tumors. The presentstudy explores the ethanolic extract of Gloriosa superba Linnas the anti-cancer agent using in vitro MTT assay and in vivoEhrlich’s ascites carcinoma (EAC) bearing Balb/c micemodels. Further, the study reports the IC50 value of the DPPHwas 1.21 µg/mL, and the IC50 of MTT assay against Hela andMCF7 was found to be 148.50 µg/mL and 147.23 µg/mLrespectively. For, in vivo study, animals were divided into fivegroups (n = 6) experimented for 14 days. The tumor wasinduced by intraperitoneal transplantation of Ehrlich’sascites carcinoma cells in Balb/c mice. The normal andcontrol group received normal saline. After 24 hours ofEhrlich’s Ascites Carcinoma transplantation, treatment wasstarted by injecting Cyclophosphamide (50 mg/kg)intraperitoneal whereas Gloriosa superba Linn extract (5mg/kg 10 mg/kg) was administrated orally. On the 15thday, mice were sacrificed to estimate hematology,biochemical estimation, and histopathology. The extractshowed a significant decrease (P 0.001) in body weight inthe treatment group compare to the control group furtherthere was amelioration in hematology, biochemicalestimation, and histopathology. From the result, it wasconcluded that the extract has a potent antitumor activity.