Glucose metabolism of dormant and heat-activated spores ofPhycomyces blakesleeanusburgeff

摘要

The metabolism of 14Cglucose has been studied inPhycomycesspores during dormancy, activation, and the initial stages of germination. Dormant spores are able to take up and metabolize exogenous glucose into different products; the major part of it goes to trehalose synthesis (up to 60 when the external glucose concentration exceeds 10-3M). During activation itself (i.e.a prolonged treatment at 50°) there is a general increase of glucose uptake and metabolism, without major changes in the relative rates of14C-label distribution in the different fractions (as compared to the metabolism of dormant spores), except for a drop in material insoluble in 80 ethanol and a still higher percentage (73) going to trehalose synthesis. In the early hours of germination we find an enhancement of the uptake and metabolism of glucose. Trehalose synthesis is practically switched off within 2 h whereas the major part of glucose (65) is metabolized to CO2and ethanol-insoluble proteinaceous material