UV-inactivated cells of Sulfolobus acidocaldarius rapidly regained viability when exposed to white light. This recovery was strictly dependent upon illumination with visible light and was not attenuated by prior dark-incubation. The kinetics of photoreactivation were determined at several temperatures and at several wavelengths of light. The results obtained in vivo were consistent with a DNA photolyase having a broad action spectrum. Photoreactivation of S. acidocaldarius apparently represents the first DNA repair process to be measured in an archaeon which grows optimally near 80 degrees C.
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