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外文期刊>Applied and Environmental Microbiology
>Improvement of multiple-stress tolerance and lactic acid production in lactococcus lactis NZ9000 under conditions of thermal stress by heterologous expression of escherichia coli dnaK
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Improvement of multiple-stress tolerance and lactic acid production in lactococcus lactis NZ9000 under conditions of thermal stress by heterologous expression of escherichia coli dnaK
The effects of nisin-induced dnaK expression in Lactococcus lactis were examined, and this expression was shown to improve stress tolerance and lactic acid fermentation efficiency. Using a nisin-inducible expression system, DnaK proteins from L. lactis (DnaK_(Lla)) and Escherichia coli (DnaK _(Eco)) were produced in L. lactis NZ9000. In comparison to a strain harboring the empty vector pNZ8048 (designated NZ-Vector) and one expressing dnaK_(Lla) (designated NZ-LDnaK), the dnaK_(Eco)-expressing strain, named NZ-EDnaK, exhibited more tolerance to heat stress at 40°C in GM17 liquid medium. The cell viability of NZ-Vector was reduced 4.6-fold after 6 h of heat treatment. However, NZ-EDnaK showed 13.5-fold increased viability under these conditions, with a very low concentration of DnaK_(Eco) production. Although the heterologous expression of dnaK_(Eco) did not effect DnaK_(Lla) production, heat treatment Increased the DnaK _(Lla) level 3.5- and 3.6-fold in NZ-Vector and NZ-EDnaK, respectively. Moreover, NZ-EDnaK showed tolerance to multiple stresses, including 3 NaCl, 5 ethanol, and 0.5 lactic acid (pH 5.47). In CMG medium, the lactate yield and the maximum lactate productivity of NZ-EDnaK were higher than the corresponding values for NZ-Vector at 30°C. Interestingly, at 40°C, these values of NZ-EDnaK were not significantly different from the corresponding values for the control strain at 30°C. Lactate dehydrogenase (LDH) activity was also found to be stable at 40°C in the presence of DnaK_(Eco) ·These findings suggest that the heterologous expression of dnaK_(Eco) enhances the quality control of proteins and enzymes, resulting in improved growth and lactic acid fermentation at high temperature.
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