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外文期刊>Applied and Environmental Microbiology
>Stereochemistry of furfural reduction by a saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification
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Stereochemistry of furfural reduction by a saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification
Ari1p from Saccharomyces cerevisiae, recently Identified as an Intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains furfural at a relatively high concentration. NADPH is Arilp's best known hydride donor. Here we report the stereochemistry of the hydride transfer step, determined by using (4R)-4-~2HNADPD and (4S)-4-~2HNADPD and unlabeled furfural in Ari1p-catalyzed reactions and following the deuterium atom into products 2-furanmethanol or NADP ~+. Analysis of the products demonstrates unambiguously that Ari1p directs hydride transfer from the si face of NADPH to the re face of furfural. The singular orientation of substrates enables construction of a model of the Michaelis complex in the Ari1p active site. The model reveals hydrophobic residues near the furfural binding site that, upon mutation, may increase specificity for furfural and enhance enzyme performance. Using (4S)-4- ~2H NADPD and NADPH as substrates, primary deuterium kinetic isotope effects of 2.2 and 2.5 were determined for the steady-state parameters k _(cat)~(NADPH) and k_(cat)/k_m~(NADPH),respectively, indicating that hydride transfer is partially rate limiting to catalysis.
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U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University Street, Peoria, IL 61604, United States;