IGFI suppresses cholesterol accumulation in the liver of growth hormone-deficient mice via the activation of ABCA1

摘要

Recently, several clinical studies have suggested that adult growth hormone (GH) deficiency that also has low concentration of IGFI is associated with an increased prevalence of fatty liver (FL). ATP-binding cassette transporter A1 (ABCAl) is a pivotal regulator of lipid efflux from cells to apolipoproteins and plays an important role on formation of FL. In this study, we determined the effects of IGFI on ABCAl expression in GH-deficient mice to clarify its effects on FL. Western blotting, real-lime PCR, and a luciferase assay were employed to examine the effect of IGFI. The binding of FoxOI to the ABCAl promoter was assessed by chromatin immunoprecipitation (ChIP) assay. Cholesterol accumulation was analyzed by Oil Red O stain and cholesterol content measurement.