Flexibility in Bioprocessing

摘要

Recent advances in bioprocessing have added a welcome dose of flexibility to producing protein therapeutics, with more rapid operations, smaller footprints, lower costs, and better separations among the wide-ranging benefits Traditionally, bioprocessing of protein therapeutics was a cumbersome, rigid operation, beginning with mammalian or microbial culture in glass and then steel tank bioreactors of increasing size. Following clarification of the culture, the protein of interest was purified via a series of glass or steel chromatographic columns containing one of a limited series of resins, as well as ultrafiltration/diafiltration and microfiltration to separate the protein from smaller components.This model succeeded in developing and producing many of the early protein therapeutics, but was inherently inefficient and inflexible.