Response of denitrification genes nirS, nirK, and nosZ to irrigation water quality in a Chinese agricultural soil

摘要

Purpose Denitrification is an important biochemical proc?ess in global nitrogen cycle, with a potent greenhouse gas product N_2O. Wastewater irrigation can result in the changes of soil properties and microbial communities of agricultural soils. The purpose of this study was to examine how the soil denitrification genes responded to different irrigation regimes. Materials and methods Soil samples were collected from three rural districts of Beijing (China) with three different irrigation regimes: clean groundwater (CW), reclaimed water (RW), and wastewater (WW). The abundance and diversity of three denitrification microbial genes (nirS, nirK, and nosZ) were examined by real-time polymerase chain reaction (PCR) and denaturing gradient gel electro?phoresis (DGGE) molecular approaches.