Gene expression profiling supports the role of Repin1 in the pathophysiology of metabolic syndrome

摘要

Congenic BB rat strains carrying a SHR segment (D4Got41-Tacr1; 60.5-122.8 Mb; BB.4S) or a WOKW segment (D4Got41-Fabp1; 60.5-104.6 Mb; BB.4W) of chromosome 4 within the BB/OK background develop facets of the metabolic syndrome when compared with their parental BB/OK rats. To narrow down potential genes involved in the pathophysiology of metabolic syndrome, gene expression studies in adipose tissues of BB/OK, BB.4S, and BB.4W rats were initiated. Total RNA of subcutaneous and epididymal adipose tissue of BB/OK (n = 10), congenic BB.4S (n = 8), and BB.4W (n = 9) males at an age of 4 weeks was isolated. The mRNA expression of 92 genes involved in obesity, insulin resistance and other metabolic traits was measured by RT-PCR. Significant differences in gene expression were only found in Repin1 in both adipose tissues. Congenic BB.4W showed significantly lower gene expression than did BB.4S and BB/OK. Our findings and newly published findings of Repin1 in 3T3-L1 adipocytes support the hypothesis that Repin1 may affect the development of facets of the metabolic syndrome.