Design, Synthesis, and Biological Evaluation of 1?Phenylpyrazolo3,4?epyrrolo3,4?gindolizine-4,6(1H,5H)?diones as New Glycogen Synthase Kinase-3β Inhibitors

摘要

Compound 5 was selected from our in-house library as a suitable starting point for the rational design of new GSK-3β inhibitors. MC/FEP calculations of 5 led to the identication of a structural class of new GSK-3β inhibitors. Compound 18 inhibited GSK-3β with an IC_(50) of 0.24 μM and inhibited tau phosphorylation in a cell-based assay. It proved to be a selective inhibitor of GSK-3 against a panel of 17 kinases and showed >10-fold selectivity against CDK2. Calculated physicochemical properties and Volsurf predictions suggested that compound 18 has the potential to diffuse passively across the blood?brain barrier.