Assessment of stability of CD34+ cell products enriched by immunoselection from peripheral blood mononuclear cells during refrigerated storage

摘要

Abstract Durable engraftment of transplanted CD34+ cells largely depends on the quality of the cell product. Limited data are currently available about extended storage of immunoselected CD34+ cells. The aim of our study was to assess the stability of CD34+ cell product with the cells stored in high concentration (80×10 6 in 6mL) in small bags intended for cell implantation. Cell products were prepared by leukapheresis and immunoselection (Clinimacs plus procedure) from 13 patients with chronic dilated cardiomyopathy. CD34+ cell products were stored at 2–8°C and analyzed at time 0 (fresh products), 24, 48h, 4 and 6 days. Product viability, absolute number of viable CD34+ cells and apoptosis were determined by flow cytometry. Microbiological contamination of the cell products was tested by BACTEC system. The mean viability of CD34+ cells decreased by 2.7 within 24h, by 13.4 within 48h and by 37.5 within 6 days. The mean recovery of viable CD34+ cells was 91.1, 74.8, 66.3 and 56.2 at 24, 48h, 4 and 6 days, respectively. The mean fraction of early apoptotic cells in fresh and stored products was 4.9±3.5 at 0h, 5.9±3.8 at 24h, 4.2±3.1 at 48h, 6.3±2.6 at 4 days and 9.3±4.6 at 6 days. All products were negative for microbial contamination. >