Suppression of gut dysbiosis reverses Western diet-induced vascular dysfunction

摘要

Vascular dysfunction represents a critical pre-clinical step in the development of cardiovascular disease. We examined the role of the gut microbiota in the development of obesity-related vascular dysfunction. Male C57BL/6J mice were fed either a standard diet (SD) (n = 12) or Western diet (WD) (n = 24) for 5 mo, after which time WD mice were randomized to receive either tin-supplemented drinking water or water containing a broad-spectrum antibiotic cocktail (WD + Abx) (n = 12/group) For 2 mo. Seven months of WD caused gut dysbiosis, increased arterial stiffness (SD 412.0 ± 6.0 vs. WD 458.3 ± 9.0 cm/s, P < 0.05) and endothelial dysfunction (28 decrease in max dilation, P < 0.05), and reduced L-NAME-inhibited dilation.