Comparison of Four Methods for the Dimethylacetal‐free Formation of Fatty Acid Methyl Esters from Phospholipids of Animal Origin

摘要

ABSTRACTFatty acid methyl esters (FAMES) were prepared from phospholipid extracts of beef, pork, lamb, chicken and turkey using four methylating procedures: BF3/methanol, KOH/BF3, direct transesterification with KOH, and a commercial preparation, Meth Prep II. Gas chromatographic analysis of the FAMES revealed the presence of dimethyl acetals (DMAs) in samples methylated with BF3/methanol or KOH/ BF3. These DMAs were evident when samples were analyzed on both polar and non‐polar columns. DMAs were not found in samples derivatized by direct alkali transesterification or by Meth Prep I