ABSTRACTVarious fixation techniques were employed in an effort to prepare a dry protein isolate from soybeans for analysis using transmission electron microscopy. A number of fixation schemes proved successful, and different properties of the material were brought out by the various treatments. Comparisons of electron micrographs of the isolate fixed and extracted in different ways yielded an effective means for determining the distribution of fat and protein in the sample and the effect of moisture on that distribution. Analysis of the isolate demonstrated the occluding effect of dry protein for fat located in walls of the protein shells of the isolate.
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