Comparison of Four Different Methods for the Analysis of Dietary Fiber in Winged Bean Seeds

摘要

ABSTRACTTwo chemical and two enzymatic procedures were evaluated with respect to efficiency of protein removal and consistency in yield and composition of soluble and insoluble dietary fiber from winged bean seeds, (cultivar TPT‐2). Considerable variability was observed in the yield and composition of the fiber fractions, but the results became more consistent when contamination of the non‐carbohydrate components (protein, ash and lignin) were accounted for in the calculations. The constituent sugars of polysaccharides were found to be similar in all four methods. Results indicated the need to critically optimize conditions to achieve efficient deproteinization and the need to differentiate “inherent” and “contaminating