ABSTRACTOptimal temperature, lower temperature limit, extent, and rate of histamine production in a tuna fish infusion broth (TFIB) varied for the strains ofProteus morganii, Klebsiella pneumoniae, Hafnia alvei, Citrobacter freundii, andEscherichia colistudied.P. morganiiandK. pneumoniaeproduced large quantities of histamine in a relatively short incubation period (2500 nmoles/ml) only at 30°C and 37°C on prolonged incubation (≥48 hr). At 72 hr of incubation, optimal temperature for histamine production was 37°C forE. coliandC freundii; 30°C forP. morganiistrain 110SC2,K. pneumoniae, andH. alvei; and 15°C forP. morganiistrain JM. The lower temperature limits for production of toxicologically significant levels of histamine in TFIB were 7°C forK. pneumoniae; 15°C for bothP. morganiistrains; and 30°C forH. alvei, C. freundi
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