Effects of Ultrasound Treatment on the Properties of Chymosin

摘要

ABSTRACTWhen chymosin was extracted by ultrasound curd tension and syneresis were significantly lower for Berridge substrate coagulated by the ultrasound‐treated chymosin than by the control. Experimental chymosin had a shorter induction period and was more heat‐sensitive than the control. Activation energy of chymosin obtained by ultrasound treatment was significantly lower than that of the control. Ultrasound treatment did not significantly change the chromatographic patterns of chymosin. Only two distinct enzymatically active proteins were observed with DEAE‐cellulose chromatography. Electrophoretic properties were similar for chymosins obtained by ultrasound and control me