Pyrene excimer incorporated into duplex DNA is an excellent probe to study protein-DNA interactions. The intense emission and ratiometric response provide high sensitivity and robustness of the probe. In the current study, by using the pyrene excimer strategy we can convert the DNA complex of the MerR protein into a ratiometric biosensor for Hg~(2+). The basis for a highly sensitive, selective, ratiometric, water compatible sensor with fast response is demonstrated. Potentially, the same approach can be applied to other members of this protein family to construct sensitive and selective biosensors for other metal ions and organic molecules.
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