ABSTRACTTwenty‐four hour postmortm intact bovine Longissimus dorsi muscle strips were (1) washed to remove sarcoplasmic fluid, or (2) left intact, and were either uninoculated or inoculated withPseudomonas fragiATCC 4973 to evaluate the effect of decreased concentration of sarcoplasm on bacterial growth and subsequent spoilage during storage at 4°C for 12 days. Washing removed the majority of the water‐soluble components of the muscle. Significantly (P<0.01) higher growth rates were observed on intact muscle than on washed muscle. Increased extractability of water‐soluble and salt‐soluble proteins was observed in intact inoculated (II) muscle as growth ofP. fragiprogressed. Alterations in SDS‐gel electrophoretic patterns of water‐soluble, salt‐soluble, urea‐soluble and urea‐insoluble proteins were evident in the II muscle. Relatively little change in nonprotein nitrogen, water‐soluble and salt‐soluble protein content occurred in washed inoculated (WI) muscle with increased bacterial growth. Only minor changes in the SDS‐gel electrophoretograms of the salt‐soluble proteins from t
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