Qualitative and Quantitative Determination of Caseins with Reverse‐Phase and Anion‐Exchange HPLC

摘要

ABSTRACTWhole native caseinate (WNC) and casein (CN) fractions from preparative DE‐52 cellulose urea columns were chromatographed using C‐8 reverse‐phase (RP) and DEAE‐type anion‐exchange (AEx) HPLC systems. With RP, αS2‐CN and κ‐CN eluted first as several small peaks; αS1‐CN eluted later as two peaks, followed by β‐CN peaks. With AEx, κ‐CN eluted early as a group of peaks, β‐CN eluted next, and αS1‐CN and αS2‐CN coeluted last. Standard curves were prepared for αS1‐CN and β‐CN using RP‐PHLC and showed correlation coefficients of 0.99 and 0.98, respectively. The caseins in WNC, nonfat dry milk casein, commercial casein(ates) and caseins from