ABSTRACTA relatively simple and efficient chromatographic method utilizing deaminated chitin (Dech), rather than chitin, has been developed and is described for the isolation, purification and concentration of lysozyme from food or biological tissues prior to quantification. It is shown that deaminated chitin has a high specificity and capacity for lysozyme (squid Dech more so than crab Dech), along with good stability and allowing fast flow rates. Studies with fresh egg white, human blood, human milk, goat milk and a commercial hen egg white lysozyme preparation showed recoveries of lysozyme greater than 99. The various isolated lysozymes were considered to be adequately pure as determined by amino acid analysis and disc gel electrophoresis.
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