Growth and Survival ofSalmonella typhimuriumat Low Temperature in Nutrient Deficient Media

摘要

ABSTRACTThe growth and survival of two strains ofSalmonella typhimutiumin nutrient‐deficient media at 7°C were studied. Cultures were propagated at 37°C, 24°C or 7°C in tryptic soy broth (TSB), inoculated into optimal, minimal and nutrient‐deficient media (TSB, 1 TSB; 1.0, 0.1 and 0.01 peptone water; 0.1 and 0.01 nonfat dry milk; and 30 glycol with and without 0.1 added milk solids) and incubated at 7°C. Numbers at 7°C declined initially when first propagated at 37°C. No decline was seen when propagated at the lower temperatures (24°C and 7°C). S.typhimuriumsurvived in all media for extended periods; one strain survived for more than 1 year at 7°C in 0.1 peptone